Notion web page12/3/2023 Top ranking no-code website builders for Notionįeeling properly introduced to Notion? Time to get better acquainted with no-code website builders for Notion. There are countless reasons to convert your Notion content into a website. Showcase your portfolio to potential employers or clients, launch a blog with your team, create, share and collaborate on your projects, etc. An interesting Notion page that you think others would find value in? Turning it into a website can help you reach a wider audience. In essence, turning Notion pages into a website can be an accessible, no-code way to share your content with others. After all, your Notion workspace can grow as your team grows. Notion offers a free version for unlimited personal use, with the possibility to upgrade to more features and functionality. Bye bye, tedious repetitive tasks, hello collaborative success! 5. Real-time updatesĪs a hub for asynchronous communication, Notion's live updates instantly reflect all changes made by any across the entire platform, eliminating the need for manual updates. You have full control over how to organize and visualize your content with a choice of six unique database views, including labels, filters, and a search option. One of the main features of the Notion 2.0 release was databases. The best part? The collection of 500+ LEGO-style building blocks, which allow you to add numerous content types like text blocks, checklists, graphs, etc., without needing to code. Notion's drag-and-drop feature makes it effortless to create new pages and add content. Notion’s Template Gallery | Source: Notion 2. Many templates are free, and others are available for a one-time fee. This means you don’t need to start from scratch each time you want to create a (web)page. One of the most convenient Notion features is its template gallery, which offers a wide range of ready-to-use, functional and well-designed templates to meet various use cases. So, why has Notion become more popular than other productivity tools? The note-taking software quickly grew into a simple, yet versatile all-in-one productivity tool to collect ideas, store large chunks of information, and-more importantly-manage projects and workflows. Notion even managed to become Product of the Month on Product Hunt in March of that year, proof that the public welcomed the update with open arms. Many factors attribute to its success, yet the release of Notion 2.0 in 2018 was one that didn’t miss its target. Capture thoughts, manage projects, or even run an entire company - and do it exactly the way you want.” | Source: Notion On the verge of failure to a roaring successĪlthough the company faced huge challenges early on, Notion today has grown into an impressive 10 billion dollar leader in productivity and collaboration, empowering its 20+ million users to be more organized. “Notion is a single space where you can think, write, and plan. On the other hand, the duo aimed to develop a tool that was more customizable for its users, with LEGO-style building blocks. This would allow users to escape an evermore fragmented workflow-the result of the many specialized, single-purpose tools that were mushrooming during that time. On the one hand, Zhao and Last wanted to create a more collaborative note-taking tool that combined the rapid advancements of computer technology. The cofounders allowed their focus to shift and become two-fold. Lack of the public’s interest and software scalability, however, threw a spanner in the works. Originally, Notion was intended as a tool to create no-code applications. In early 2013, Ivan Zhao and Simon Last founded the San Francisco-based software company Notion. Next, we will help you decide on your favorite no-code website builder for Notion with a top 5 of our own. In this blog post, we will first explain why Notion is an excellent all-in-one productivity tool to build websites-such as real estate listings, job boards, blog overview, etc. You may not, however, know how relatively easy it is to convert Notion pages into your very own website. Have you ever wanted to boost your productivity? Chances are you have heard about Notion.
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Klenow fragment pdf12/3/2023 Preparation of radioactive DNA probes: Examine each of the reactions depicted above. This reaction is more efficienty conducted with T4 DNA polymerase, which has much more potent exonuclease activity. Removal of nucleotides from the 3' ends will continue, but, in the presence of nucleotides, the polymerase activity will balance the exonuclease activity, yielding blunt ends. The 3' -> 5' exonuclease activity of Klenow will digest away the protruding overhang. This reaction is conceptually identical to the one described above, but with a huge primer and a very short segment of single-stranded template.ĭigesting away protruding 3' overhangs: This is another method for producing blunt ends on DNA, in this with ends generated from restriction enzymes that cleave to produce 3' overhangs. One item of some significance in the above reaction is that as Klenow proceeds, it can displace primers downstream and continue synthesizing new DNA.įilling in recessed 3' ends of DNA fragments: A "fill-in" reaction is used to create blunt ends on fragments created by cleavage with restriction enzymes that leave 5' overhangs. The reaction proceeds are depicted below: To use Klenow to synthesize a complementary strand of DNA, one simply mixes single-stranded template (usually denatured double-standed DNA), primers and the enzyme in the presence of an appropriate buffer (most restriction enzyme buffers work well). The oligonucleotides must be complemenary to some section of template DNA. The primers used for most in vitro polymerization reactions are single-stranded DNAs, typically 6 to 20 bases in length, called oligonucleotides. Performing that task in the lab is integral to such processes as synthesizing the second strand DNA in cDNA cloning and generating radioactive probes for hybridization reactions.ĭNA polymerases require a primer to provide a free 3' hydroxyl group for initiation of synthesis. Synthesis of double-stranded DNA from single-stranded templates: The function of DNA polymerases is to synthesize complementary strands during DNA replication. Klenow fragment is useful for several tasks: The enzyme you purchase is almost certainly produced in this manner. In addition to generating Klenow fragment by proteolysis, it can be expressed in bacteria from a truncated form of the DNA polymerase I gene. The large or Klenow fragment of DNA polymerase I has DNA polymerase and 3' -> 5' exonuclease activities, and is widely used in molecular biology. Exposure of DNA polymerase I to the protease subtilisin cleaves the molecule into a small fragment, which retains the 5' -> 3' exonuclease activity, and a large piece called Klenow fragment. However, this pesky enzymatic activity can readily be removed from the holoenzyme. coli's DNA polymerase I makes it unsuitable for many applications. Using a PowerMax AC to DC Converter Charger With Battle Born Batteries Features Recommended by many of the popular Lithium Battery Manufacturers such as Battle Born, ReLion, Lithioncs, Life Blue, and more! Need more power, our 12V converters can be Paralleled to provide even more charging power for those of you who need to charge FAST and play HARD We offer several different amperage sizes to fit all requirements! A 100 Amp charger will charge a 12V 100Ah battery in approximately 1 hour, and a 200Ah battery bank in 2 hours. Need to charge a large bank quickly between uses for RV, Automotive, Car Audio, Off-Grid, Bowfishing, or Communications systems. 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By placing this order you agree to be solely responsible for liability for special, direct, indirect, consequential or punitive damages caused by the use, storage and misuse of products sold by or available from Advance MCS Electronics, Inc.The PowerMax PM3 Series converters 110Vac power to 12Vdc power to charge a battery and/or power 12V equipment. 3 stage charger PLUS desulfation stage Built in Smart Charger Current limiting design automatically shuts down power during overload or short-circuit conditions Reverse Battery Protection UL and CUL Approved 13.2 Vdc range "float" mode 13.6 Vdc range "absorption" mode 14.4 Vdc range "bulk" mode Check our other listings for all available sizes: 35, 45, 55, 75 AND 100 AMP MODELS Features In no event shall Advance MCS Electronics, Inc., it's directors, officers, employees and representatives be liable for special, direct, indirect, consequential or punitive damages related to products sold or available from Advance MCS Electronics, Inc., even if made aware of the possibilty of such damages. Use on your test bench to keep battery banks topped off or in your automotive shop to send 12v DC power to the vehicle. DONE! Great for RV's or anywhere you need 12v DC power with high amperage. DC output is via junction set screw style terminal block: -positive (red) -negative (black) Simply strip your heavy gauge battery charge wires and insert into the junction and tighten firmly. 75 amp 110v to 12vdc power factor converter charger PM4-75 110 volt to 12 volt Power Converter (75 amps max) Description Looking for a replacement converter for your RV and don't want to spend hundreds of dollars at your local RV center? ************Install this yourself and save a bundle*********** AC TO DC CONVERTER / CHARGER Beefy unit that's painfully simple to install with built in mounting flanges and an AC power cord to plug into standard 110/120v outlet. Great for RV's or anywhere you need 12v DC power with high amperage. Item: 371231983244 PowerMax PM4-75 amp RV Power Converter 12 vdc volt DC Battery charger maintainer. Ships to: WORLDWIDE & many other countries, Designer cat eye reading glasses12/3/2023 Sam’s Club provides a broad selection of designer reading glasses and frames to fit with your doctor’s prescription. When you’re shopping, here are the main categories of eye products and eyewear you are likely to come across in your search: Reading glasses Recommended Reading: How To Measure Pupil Distance For Glasses What Different Types Of Eyewear And Eye Products Are There App Store is a service mark of Apple Inc. All rights reserved.Īndroid, Google Play and the Google Play logo are trademarks of Google Inc. KOHLS® and Kohls brand names are trademarks owned by KIN, Inc. Scan the code to downloadthe Kohls App today. Promotional offers available online at may vary from those offered in Kohl’s stores. 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When you purchase Duplicate Sweeper you are purchasing a single user license for 1 computer. By registering this program you declare the product to be fit for your purposes. Following clearance of payment you become a registered owner of this product. Upon completion of your evaluation of the product you agree to either (a) Uninstall the software or (b) purchase an activation code to convert the trial program into a full registered copy of the product. You are entitled to download this product and use it evaluation purposes. THIS INCLUDES BUT IS NOT EXCLUSIVE TO THE LOSS OF DATA OR DAMAGE TO ANY EQUIPMENT. NEITHER THE AUTHOR NOR ANYONE ELSE WHO HAS BEEN INVOLVED IN THE CREATION, PRODUCTION OR DELIVERY OF THIS PRODUCT SHALL BE LIABLE FOR ANY DIRECT, INDIRECT, CONSEQUENTIAL OR INCIDENTAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE SUCH PRODUCT EVEN IF THE AUTHOR HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. THIS PRODUCT AND ACCOMPANYING WRITTEN MATERIALS ARE PROVIDED "AS IS" WITHOUT WARRANTIES OF ANY KIND, EITHER EXPRESS OR IMPLIED, INCLUDING, BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. If at any time the terms and conditions of this Agreement are no longer acceptable to you, you should immediately cease all use of the product. Your continued use of the product constitutes your binding acceptance of these terms and conditions, including any changes or modifications made by the authors as permitted above. The authors’ reserve the right, at their discretion, to change or modify all or any part of this Agreement at any time, effective immediately upon notice published on If you do not agree to the terms and conditions of this agreement promptly remove the product from your computer. Duplicate Sweeper and any of its supporting documentation are herein after referred to as the product.īy installing or using the product, you indicate your complete and unconditional acceptance of these terms and conditions. This license agreement is a legal agreement between you the end user (either as an individual or an entity) and the authors of Duplicate Sweeper (Wide Angle Software Ltd) herein after referred to as the authors. Whats a caffeine overdose12/2/2023 If someone does overdo their caffeine intake and takes too much caffeine regularly, studies have suggested that this may cause adrenal glands to become weakened which can cause sickness. This is due to the fact that caffeine is a powerful sleep-deterrent and sleep is essential at this stage in a child's growth and development.Īccording to the European Food Safety Authority, adults shouldn’t consume more than the aforementioned 400mg of caffeine a day in daily life, as exceeding this could lead to increased heart rate, higher blood pressure, irregular heartbeat, tremors, nervousness, insomnia, and panic attacks. Learn more about how to use caffeine DURING exercise of different durations.įor those under the age of 12, caffeine consumption isn’t recommended, and for those aged 12 to 18 it should be limited to no more than ~100-200 mg per day (or 2.5 mg per kilogram of bodyweight). Caffeine SourceĬaffeinated Energy Drink (per 500ml/16oz) So, for a 70kg / 150lb athlete that’d be in the region of ~200-400mg) in the final 60 minutes before exercise lasting 4 hours or less, as well as topping up on caffeine during exercise that goes beyond 4 hours in duration. If caffeine is likely to be beneficial to you, the science recommends taking a caffeine dose of ~3-6mg per kilogram of bodyweight ( 1.4-2.7mg/lbs). When it comes to the daily recommendations for adults, ~400mg of caffeine (about 4 coffees a day) appears to be a safe daily dosage for most, although pregnant women should err on the side of caution at ~200mg a day, as a study found higher dosages may be linked to low birth weights.įor athletes considering using caffeine to improve their performance during exercise, it’s a good idea to first answer the question: is caffeine right for you? by completing this self-scoring system. Ultimately, your experience of the effects of caffeine and exactly how much is too much will be individual to you and be influenced by your habitual use, tolerance and sensitivity… How much is too much? You can probably relate to the need for a morning coffee to kick-start your day, or a caffeine gel to get you through to the finish line of a race, but can you have too much of a good thing?Ĭaffeine is proven to keep fatigue at bay and boost perceived energy levels in most people when taken in moderation, but there can be negative side-effects when taking too much.įrom the relatively ‘minor’ effects of headaches, jitters and sleep deprivation after drinking one ‘cup of joe’ too many, to more extreme and rare cases where a massive caffeine intake has unfortunately proven to be fatal. Parents newsletters12/2/2023 Years ago I had a boss who frequently used the slogan “six weeks, six ways,” to describe effective ministry communication. I pray you’ll be encouraged to pursue these same goals in your own communication, and that the following will provide practical help in doing so.Ĭonvey Upcoming Events and Ministry Details With this in mind, there are three things I hope to accomplish in each of our weekly newsletters to parents. Youth ministers occupy a critical middle space between teenagers and parents: Whether we are parents ourselves or not, simply being adults means we can empathize with the concerns and questions parents face meanwhile, as people who have devoted our lives to discipling younger people, we have a wide range of experiences that can help us interpret what teenagers are going through. So a regular communication sent right to their inboxes not only shows that their youth minister is thinking about them, it is an it is an easy way to provide them with needed resources. They may feel they’re parenting on fumes, just getting by from day to day. At the same time, parents today find themselves without a lot of time to read parenting books or search the web for sound resources. Many of them feel like they are regularly failing, and fear is a constant companion for almost all. In my experience, parents are looking for voices that will encourage and equip them to parent through the tumultuous teenage years. It’s a time when I pray for students and their parents, calling to mind the events and conversations of the past week and looking for themes to highlight or explore. Over the past number of years, creating this email has become something of a spiritual discipline for me. A regular rhythm that has dramatically increased my communication with and ministry to parents of youth has been the habit of sending a weekly newsletter. Leggy seedlings fix12/2/2023 When pruning basil, it’s important to use clean, sharp scissors or pruning shears to avoid damaging the plant. This will redirect the plant’s energy away from producing seeds and towards growing more leaves. If your basil has already started to flower, you should also remove the flower buds. You can repeat this process every few weeks, depending on the size of your plant. This will encourage your basil to branch out and grow bushier. Once your plant has grown to this size, it’s time to start pruning.īegin by pinching off the top two sets of leaves on each stem. The first thing to keep in mind is to avoid pruning your basil until it has at least six leaves. Pruning basil is an essential part of growing healthy and productive plants. If that is the case you can still use organic fertilizer that you should be able to get at most gardening stores. The best fertilizer will always be manure be sometimes it can be hard getting a hold of it, or the fact it does not exactly smell like roses makes it appealing to use. This amount won’t overdo it and you will not accidentally stress the plant too much. We usually recommend fertilizing the soil about 4 times a year. Consider therefore using fertilizer a few times during the year. Basil is a plant that does really like healthy soil. It can also be because of too little nutrition in the soil. This way you can ensure the continued growth and a bushy plant.īut the sun might not be the sole reason all the time. So if you are having issues getting a sufficient amount you should consider moving it outdoors perhaps. If you keep it outdoors however this number goes down to 6 – 8 hours. Basil growing indoors will need up to 10 hours of light to need to be a leggy plant. Just like we said at the beginning of this article, the biggest reason for your basil growing the way it does is the limited access to the sun it’s getting. It talks about making the most of the harvest, find it here, How To Preserve And Freeze Thai Basil. If you are growing a lot of Thai basil at home and don’t know what to do with all of it then we have the perfect guide for you. It’s not too late, even though it might be near the end of the season. In the article, we will discuss some of the measures you can take to fix your plant. But it can also be because of the health of the soil. Otherwise, it will grow this way, unfortunately. It needs many hours to really promote growth in a bushy way. One of the biggest reasons for this happening is that it’s not getting enough sun during the day. It can be very annoying to have the basil grow this way. Welcome to this post about how you can fix a leggy basil plant. Home » Edible Garden » Herbs » Basil » How To Fix Leggy Basil How To Fix Leggy Basil – How To Prune Basil – Can I Fix Leggy Basil – How To Fix Leggy Italian Basil – How To Fix Leggy Basil Seedlings – Can You Bury Leggy Basil Seedlings Advice to a bride on her wedding day12/2/2023 Wishing you all the best for your future. All your memories will always be in our heart like the season of spring. Soon you have to shift to your partner’s home and spend your whole life there.May your life give you every reason to smile and happiness. You are looking so amazing in this wonderful outfit of bride.I wish you all the happiness in the world dear. You look beautiful in your wedding gown darling, I hope he knows how lucky he is to be having you as a wife, you are a gem and anyone who knows you can say that for a fact.Wishing you a blissful married life ahead. May the two of you bonded in everlasting love.I wish you happiness, today, now and forever. I will always be here for you if you need me, but for today, put on that wonderful smile for you are marrying the man you fell in love with.Don’t hold anything back, love endlessly give without wanting anything in return.Wishing a very happy married life with lots of joy, pleasure and good memories.May your home be an example to the kind of home every bride would dream of having. Happy married life to this beautiful bride.Most Popular: 200+ Wedding Wish to Write in a Wedding Card It’s a wonderful day to be celebrating two very wonderful people, I wish you nothing but the best as you embark on this wonderful journey together.Have a great day my dear, you deserve the best, truly.
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